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c33a hpv human cervical cancer cell line  (ATCC)


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    ATCC c33a hpv human cervical cancer cell line
    HPV16E7 expression promotes actin cytoskeleton remodeling, cell invasion ability and EMT in human cervical cancer cells. ( A ) IVM analysis after double-cell staining with TRITC-phalloidin (red) and Hoechst (blue) in <t>C33A</t> cells transfected (green) with pAmCyan empty vector (Mock), pAmCyan-HPV16E7 wild-type (E7wt) or deletion (E7mut) mutant constructs pAmCyan-HPV16E7Δ62–66 (E7Δ62–66) or pAmCyan-HPV17E7Δ71–75 (E7Δ71–75). E7wt-expressing cells show different morphological features compared to both Mock- and E7 mutant transfected cells. Arrow indicates protrusive and invasive structures (left). Flow cytometry evaluation of the intracellular amount of F-actin restricted to pAmCyan-positive cells. Histograms obtained in a representative experiment are shown. Bar graph shows the mean ± SD of the median fluorescence intensity obtained in four different experiments. ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells (right). ( B ) Representative images of transwell invasion assay of pAmCyan C33A transfected cells (left). Fluorescence emission of C33A migrating through Matrigel are quantified by IVM analysis and expressed as percentage (right). ( C ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail. β−actin determination was used as loading controls. Bar graph (bottom) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ±SD. ** p < 0.01 vs. Mock transfected cells. Uncropped western blot figure available in . ( D ) Bar graph showing the evaluation of mRNA levels of Zeb1 and Snail performed by qRT-PCR assay. Data are reported as mean ±SD of RNAs relative fold change vs Mock-transfected cells obtained in three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells.
    C33a Hpv Human Cervical Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c33a hpv human cervical cancer cell line/product/ATCC
    Average 96 stars, based on 1113 article reviews
    c33a hpv human cervical cancer cell line - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "Physical Interaction between HPV16E7 and the Actin-Binding Protein Gelsolin Regulates Epithelial-Mesenchymal Transition via HIPPO-YAP Axis"

    Article Title: Physical Interaction between HPV16E7 and the Actin-Binding Protein Gelsolin Regulates Epithelial-Mesenchymal Transition via HIPPO-YAP Axis

    Journal: Cancers

    doi: 10.3390/cancers13020353

    HPV16E7 expression promotes actin cytoskeleton remodeling, cell invasion ability and EMT in human cervical cancer cells. ( A ) IVM analysis after double-cell staining with TRITC-phalloidin (red) and Hoechst (blue) in C33A cells transfected (green) with pAmCyan empty vector (Mock), pAmCyan-HPV16E7 wild-type (E7wt) or deletion (E7mut) mutant constructs pAmCyan-HPV16E7Δ62–66 (E7Δ62–66) or pAmCyan-HPV17E7Δ71–75 (E7Δ71–75). E7wt-expressing cells show different morphological features compared to both Mock- and E7 mutant transfected cells. Arrow indicates protrusive and invasive structures (left). Flow cytometry evaluation of the intracellular amount of F-actin restricted to pAmCyan-positive cells. Histograms obtained in a representative experiment are shown. Bar graph shows the mean ± SD of the median fluorescence intensity obtained in four different experiments. ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells (right). ( B ) Representative images of transwell invasion assay of pAmCyan C33A transfected cells (left). Fluorescence emission of C33A migrating through Matrigel are quantified by IVM analysis and expressed as percentage (right). ( C ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail. β−actin determination was used as loading controls. Bar graph (bottom) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ±SD. ** p < 0.01 vs. Mock transfected cells. Uncropped western blot figure available in . ( D ) Bar graph showing the evaluation of mRNA levels of Zeb1 and Snail performed by qRT-PCR assay. Data are reported as mean ±SD of RNAs relative fold change vs Mock-transfected cells obtained in three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells.
    Figure Legend Snippet: HPV16E7 expression promotes actin cytoskeleton remodeling, cell invasion ability and EMT in human cervical cancer cells. ( A ) IVM analysis after double-cell staining with TRITC-phalloidin (red) and Hoechst (blue) in C33A cells transfected (green) with pAmCyan empty vector (Mock), pAmCyan-HPV16E7 wild-type (E7wt) or deletion (E7mut) mutant constructs pAmCyan-HPV16E7Δ62–66 (E7Δ62–66) or pAmCyan-HPV17E7Δ71–75 (E7Δ71–75). E7wt-expressing cells show different morphological features compared to both Mock- and E7 mutant transfected cells. Arrow indicates protrusive and invasive structures (left). Flow cytometry evaluation of the intracellular amount of F-actin restricted to pAmCyan-positive cells. Histograms obtained in a representative experiment are shown. Bar graph shows the mean ± SD of the median fluorescence intensity obtained in four different experiments. ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells (right). ( B ) Representative images of transwell invasion assay of pAmCyan C33A transfected cells (left). Fluorescence emission of C33A migrating through Matrigel are quantified by IVM analysis and expressed as percentage (right). ( C ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail. β−actin determination was used as loading controls. Bar graph (bottom) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ±SD. ** p < 0.01 vs. Mock transfected cells. Uncropped western blot figure available in . ( D ) Bar graph showing the evaluation of mRNA levels of Zeb1 and Snail performed by qRT-PCR assay. Data are reported as mean ±SD of RNAs relative fold change vs Mock-transfected cells obtained in three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells.

    Techniques Used: Expressing, Staining, Transfection, Plasmid Preparation, Mutagenesis, Construct, Flow Cytometry, Fluorescence, Transwell Invasion Assay, Western Blot, Quantitation Assay, Quantitative RT-PCR

    Effect of Cytochalasin D on p-YAP/AMOT1 interaction and expression of EMT markers. ( A ) Quantitative evaluation of p-YAP/AMOT1 molecular association by FRET technique, as revealed by flow cytometry analysis restricted to pAmCyan-positive cells treated or not with CytoD (1 mM for 4 h). Numbers indicate the percentage of FL3 positive events obtained in one experiment representative of three. Bar graphs on the right show FRET efficiency calculated according to the Riemann’s algorithm. Data are reported as mean ± SD from three independent experiments. ** p < 0.01 vs. Mock transfected cells. ( B ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail in C33A expressing E7wt or E7 mutated treated with CytoD. β-actin determination was used as loading controls. Bar graph (right) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ± SD. ** p < 0.01, *** p < 0.001 vs the same sample treated with CytoD. Uncropped western blot images for B are available in .
    Figure Legend Snippet: Effect of Cytochalasin D on p-YAP/AMOT1 interaction and expression of EMT markers. ( A ) Quantitative evaluation of p-YAP/AMOT1 molecular association by FRET technique, as revealed by flow cytometry analysis restricted to pAmCyan-positive cells treated or not with CytoD (1 mM for 4 h). Numbers indicate the percentage of FL3 positive events obtained in one experiment representative of three. Bar graphs on the right show FRET efficiency calculated according to the Riemann’s algorithm. Data are reported as mean ± SD from three independent experiments. ** p < 0.01 vs. Mock transfected cells. ( B ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail in C33A expressing E7wt or E7 mutated treated with CytoD. β-actin determination was used as loading controls. Bar graph (right) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ± SD. ** p < 0.01, *** p < 0.001 vs the same sample treated with CytoD. Uncropped western blot images for B are available in .

    Techniques Used: Expressing, Flow Cytometry, Transfection, Western Blot, Quantitation Assay



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    c33a hpv human cervical cancer cell line  (ATCC)
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    ATCC c33a hpv human cervical cancer cell line
    HPV16E7 expression promotes actin cytoskeleton remodeling, cell invasion ability and EMT in human cervical cancer cells. ( A ) IVM analysis after double-cell staining with TRITC-phalloidin (red) and Hoechst (blue) in <t>C33A</t> cells transfected (green) with pAmCyan empty vector (Mock), pAmCyan-HPV16E7 wild-type (E7wt) or deletion (E7mut) mutant constructs pAmCyan-HPV16E7Δ62–66 (E7Δ62–66) or pAmCyan-HPV17E7Δ71–75 (E7Δ71–75). E7wt-expressing cells show different morphological features compared to both Mock- and E7 mutant transfected cells. Arrow indicates protrusive and invasive structures (left). Flow cytometry evaluation of the intracellular amount of F-actin restricted to pAmCyan-positive cells. Histograms obtained in a representative experiment are shown. Bar graph shows the mean ± SD of the median fluorescence intensity obtained in four different experiments. ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells (right). ( B ) Representative images of transwell invasion assay of pAmCyan C33A transfected cells (left). Fluorescence emission of C33A migrating through Matrigel are quantified by IVM analysis and expressed as percentage (right). ( C ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail. β−actin determination was used as loading controls. Bar graph (bottom) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ±SD. ** p < 0.01 vs. Mock transfected cells. Uncropped western blot figure available in . ( D ) Bar graph showing the evaluation of mRNA levels of Zeb1 and Snail performed by qRT-PCR assay. Data are reported as mean ±SD of RNAs relative fold change vs Mock-transfected cells obtained in three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells.
    C33a Hpv Human Cervical Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human cervical cancer cell lines c33a hpv negative  (ATCC)
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    ATCC human cervical cancer cell lines c33a hpv negative
    Figure 1. SIX1 expression is increased by <t>HPV</t> oncoprotein in cervical cancer. (A) Immunohistochemical analysis of SIX1 protein in tissue microarray of human specimens. Representative samples of SIX1 staining are shown at x200 magnification (left). Bar, 100 µm. The immunohistochemical scores (HSCORE) of SIX1 are also shown (right). (B) The expressions of SIX1 in three cervical cancer cell lines were detected by western blot analysis. (C) The expressions of HPV-E6, HPV-E7 and SIX1 in indicated <t>C33a</t> cells were detected by western blot analysis. (D) At 48 h after HPV-E7 siRNA transfection, the expressions of HPV-E7 and SIX1 in cells detected by western blot analysis. *P<0.05; **P<0.01; ***P<0.001.
    Human Cervical Cancer Cell Lines C33a Hpv Negative, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    HPV16E7 expression promotes actin cytoskeleton remodeling, cell invasion ability and EMT in human cervical cancer cells. ( A ) IVM analysis after double-cell staining with TRITC-phalloidin (red) and Hoechst (blue) in C33A cells transfected (green) with pAmCyan empty vector (Mock), pAmCyan-HPV16E7 wild-type (E7wt) or deletion (E7mut) mutant constructs pAmCyan-HPV16E7Δ62–66 (E7Δ62–66) or pAmCyan-HPV17E7Δ71–75 (E7Δ71–75). E7wt-expressing cells show different morphological features compared to both Mock- and E7 mutant transfected cells. Arrow indicates protrusive and invasive structures (left). Flow cytometry evaluation of the intracellular amount of F-actin restricted to pAmCyan-positive cells. Histograms obtained in a representative experiment are shown. Bar graph shows the mean ± SD of the median fluorescence intensity obtained in four different experiments. ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells (right). ( B ) Representative images of transwell invasion assay of pAmCyan C33A transfected cells (left). Fluorescence emission of C33A migrating through Matrigel are quantified by IVM analysis and expressed as percentage (right). ( C ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail. β−actin determination was used as loading controls. Bar graph (bottom) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ±SD. ** p < 0.01 vs. Mock transfected cells. Uncropped western blot figure available in . ( D ) Bar graph showing the evaluation of mRNA levels of Zeb1 and Snail performed by qRT-PCR assay. Data are reported as mean ±SD of RNAs relative fold change vs Mock-transfected cells obtained in three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells.

    Journal: Cancers

    Article Title: Physical Interaction between HPV16E7 and the Actin-Binding Protein Gelsolin Regulates Epithelial-Mesenchymal Transition via HIPPO-YAP Axis

    doi: 10.3390/cancers13020353

    Figure Lengend Snippet: HPV16E7 expression promotes actin cytoskeleton remodeling, cell invasion ability and EMT in human cervical cancer cells. ( A ) IVM analysis after double-cell staining with TRITC-phalloidin (red) and Hoechst (blue) in C33A cells transfected (green) with pAmCyan empty vector (Mock), pAmCyan-HPV16E7 wild-type (E7wt) or deletion (E7mut) mutant constructs pAmCyan-HPV16E7Δ62–66 (E7Δ62–66) or pAmCyan-HPV17E7Δ71–75 (E7Δ71–75). E7wt-expressing cells show different morphological features compared to both Mock- and E7 mutant transfected cells. Arrow indicates protrusive and invasive structures (left). Flow cytometry evaluation of the intracellular amount of F-actin restricted to pAmCyan-positive cells. Histograms obtained in a representative experiment are shown. Bar graph shows the mean ± SD of the median fluorescence intensity obtained in four different experiments. ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells (right). ( B ) Representative images of transwell invasion assay of pAmCyan C33A transfected cells (left). Fluorescence emission of C33A migrating through Matrigel are quantified by IVM analysis and expressed as percentage (right). ( C ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail. β−actin determination was used as loading controls. Bar graph (bottom) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ±SD. ** p < 0.01 vs. Mock transfected cells. Uncropped western blot figure available in . ( D ) Bar graph showing the evaluation of mRNA levels of Zeb1 and Snail performed by qRT-PCR assay. Data are reported as mean ±SD of RNAs relative fold change vs Mock-transfected cells obtained in three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Mock transfected cells.

    Article Snippet: C33A (HPV-) human cervical cancer cell line (American Type Culture Collection ATCC; Rockville, MD, USA) was cultured in DMEM (Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10% FBS (Euroclone, Milan, Italy).

    Techniques: Expressing, Staining, Transfection, Plasmid Preparation, Mutagenesis, Construct, Flow Cytometry, Fluorescence, Transwell Invasion Assay, Western Blot, Quantitation Assay, Quantitative RT-PCR

    Effect of Cytochalasin D on p-YAP/AMOT1 interaction and expression of EMT markers. ( A ) Quantitative evaluation of p-YAP/AMOT1 molecular association by FRET technique, as revealed by flow cytometry analysis restricted to pAmCyan-positive cells treated or not with CytoD (1 mM for 4 h). Numbers indicate the percentage of FL3 positive events obtained in one experiment representative of three. Bar graphs on the right show FRET efficiency calculated according to the Riemann’s algorithm. Data are reported as mean ± SD from three independent experiments. ** p < 0.01 vs. Mock transfected cells. ( B ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail in C33A expressing E7wt or E7 mutated treated with CytoD. β-actin determination was used as loading controls. Bar graph (right) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ± SD. ** p < 0.01, *** p < 0.001 vs the same sample treated with CytoD. Uncropped western blot images for B are available in .

    Journal: Cancers

    Article Title: Physical Interaction between HPV16E7 and the Actin-Binding Protein Gelsolin Regulates Epithelial-Mesenchymal Transition via HIPPO-YAP Axis

    doi: 10.3390/cancers13020353

    Figure Lengend Snippet: Effect of Cytochalasin D on p-YAP/AMOT1 interaction and expression of EMT markers. ( A ) Quantitative evaluation of p-YAP/AMOT1 molecular association by FRET technique, as revealed by flow cytometry analysis restricted to pAmCyan-positive cells treated or not with CytoD (1 mM for 4 h). Numbers indicate the percentage of FL3 positive events obtained in one experiment representative of three. Bar graphs on the right show FRET efficiency calculated according to the Riemann’s algorithm. Data are reported as mean ± SD from three independent experiments. ** p < 0.01 vs. Mock transfected cells. ( B ) Western blot analysis of the expression of the EMT markers Zeb1 and Snail in C33A expressing E7wt or E7 mutated treated with CytoD. β-actin determination was used as loading controls. Bar graph (right) shows relative densitometry quantitation of each protein normalized to β-actin obtained in three independent experiments and reported as mean ± SD. ** p < 0.01, *** p < 0.001 vs the same sample treated with CytoD. Uncropped western blot images for B are available in .

    Article Snippet: C33A (HPV-) human cervical cancer cell line (American Type Culture Collection ATCC; Rockville, MD, USA) was cultured in DMEM (Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10% FBS (Euroclone, Milan, Italy).

    Techniques: Expressing, Flow Cytometry, Transfection, Western Blot, Quantitation Assay

    Figure 1. SIX1 expression is increased by HPV oncoprotein in cervical cancer. (A) Immunohistochemical analysis of SIX1 protein in tissue microarray of human specimens. Representative samples of SIX1 staining are shown at x200 magnification (left). Bar, 100 µm. The immunohistochemical scores (HSCORE) of SIX1 are also shown (right). (B) The expressions of SIX1 in three cervical cancer cell lines were detected by western blot analysis. (C) The expressions of HPV-E6, HPV-E7 and SIX1 in indicated C33a cells were detected by western blot analysis. (D) At 48 h after HPV-E7 siRNA transfection, the expressions of HPV-E7 and SIX1 in cells detected by western blot analysis. *P<0.05; **P<0.01; ***P<0.001.

    Journal: International journal of oncology

    Article Title: Sine oculis homeobox homolog 1 promotes DNA replication and cell proliferation in cervical cancer.

    doi: 10.3892/ijo.2014.2510

    Figure Lengend Snippet: Figure 1. SIX1 expression is increased by HPV oncoprotein in cervical cancer. (A) Immunohistochemical analysis of SIX1 protein in tissue microarray of human specimens. Representative samples of SIX1 staining are shown at x200 magnification (left). Bar, 100 µm. The immunohistochemical scores (HSCORE) of SIX1 are also shown (right). (B) The expressions of SIX1 in three cervical cancer cell lines were detected by western blot analysis. (C) The expressions of HPV-E6, HPV-E7 and SIX1 in indicated C33a cells were detected by western blot analysis. (D) At 48 h after HPV-E7 siRNA transfection, the expressions of HPV-E7 and SIX1 in cells detected by western blot analysis. *P<0.05; **P<0.01; ***P<0.001.

    Article Snippet: Human cervical cancer cell lines C33a (HPV-negative), Siha and Caski (HPV-positive) were purchased from the American Type Culture Collection (Manassas, VA).

    Techniques: Expressing, Immunohistochemical staining, Microarray, Staining, Western Blot, Transfection

    Figure 3. SIX1 regulates the expression of genes responsible for DNA replication. (A and B) In C33a-3.1 and C33a-SIX1 cells, SIX1 expression was detected by (A) western blot analysis, and the expression levels of indicated genes were detected by (B) real-time RT-PCR. (C and D) Two independent shRNAs were used to knockdown SIX1 expression in Caski cells. SIX1 expression was detected by (C) western blot analysis and the expression levels of indicated genes were detected by (D) real-time RT-PCR. *P<0.05; **P<0.01; ***P<0.001.

    Journal: International journal of oncology

    Article Title: Sine oculis homeobox homolog 1 promotes DNA replication and cell proliferation in cervical cancer.

    doi: 10.3892/ijo.2014.2510

    Figure Lengend Snippet: Figure 3. SIX1 regulates the expression of genes responsible for DNA replication. (A and B) In C33a-3.1 and C33a-SIX1 cells, SIX1 expression was detected by (A) western blot analysis, and the expression levels of indicated genes were detected by (B) real-time RT-PCR. (C and D) Two independent shRNAs were used to knockdown SIX1 expression in Caski cells. SIX1 expression was detected by (C) western blot analysis and the expression levels of indicated genes were detected by (D) real-time RT-PCR. *P<0.05; **P<0.01; ***P<0.001.

    Article Snippet: Human cervical cancer cell lines C33a (HPV-negative), Siha and Caski (HPV-positive) were purchased from the American Type Culture Collection (Manassas, VA).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Knockdown